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Purification and preparation of Rhodobacter sphaeroides reaction centers for photocurrent measurements and atomic force microscopy characterization

Summary: The formation of defined surfaces consisting of photosynthetic reaction centers (RCs) in biohybrid solar cells is challenging. Here, we start with the production of engineered RCs for oriented binding. RCs are deposited onto gold electrodes, and 6-mercapto-1-hexanol (MCH) is used to displac...

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Principais autores: Daniel Jun, Sylvester Zhang, Adrian Jan Grzędowski, Amita Mahey, J. Thomas Beatty, Dan Bizzotto
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2022-03-01
Serier:STAR Protocols
Fag:
Biophysics
Atomic Force Microscopy (AFM)
Microbiology
Microscopy
Protein Biochemistry
Protein expression and purification
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166721007504
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