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Rapid quantitation of NAD+/NADH and NADPH/NADP+ with mass spectrometry by using calibration constants

NAD+/NADH and NADPH/NADP+ are important indicators of cellular redox status, but they cannot be reliably calculated from the relative intensities of mass spectrometry signals alone. Establishing accurate redox ratios by mass spectrometry has historically required converting relative signal intensiti...

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Principais autores: Qiuyuan Guo, Mike Lingjue Wang, Michaela Schwaiger-Haber, Xiangfeng Niu, Shanshan Zhang, Leah P. Shriver, Gary J. Patti
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2026-02-01
Series:Redox Biology
Assuntos:
Nicotinamide adenine dinucleotide
Nicotinamide adenine dinucleotide phosphate
NAD+/NADH
NADPH/NADP+
LC/MS
Calibration constants
Acceso en liña:http://www.sciencedirect.com/science/article/pii/S2213231725004665
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