Protocol for transcriptomic and epigenomic analyses of tip-like endothelial cells using scRNA-seq and ChIP-seq

Summary: Angiogenesis begins as endothelial cells migrate, forming a sprouting tip and subsequent growth-rich stalk cells. Here, we present a protocol for transcriptomic and epigenomic analyses of tip-like cells in cultured endothelial cells. We describe steps for stimulating human umbilical vein en...

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Autores principales:	Shintaro Funasaki, Yuri Miyamura, Shunsuke Kamei, Akhinur Rahman, Masaya Yamazaki, Shingo Usuki, Keiichiro Yasunaga, Yorifumi Satou, Hiroto Ohguchi, Takashi Minami
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Elsevier 2025-03-01
Colección:	STAR Protocols
Materias:	Bioinformatics Sequence analysis Single Cell Genomics RNAseq ChIPseq
Acceso en línea:	http://www.sciencedirect.com/science/article/pii/S266616672400491X
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