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Agrobacterium-Mediated Genetic Transformation of Embryogenic Callus in a Liriodendron Hybrid (L. Chinense × L. Tulipifera)

A highly efficient genetic transformation system of Liriodendron hybrid embryogenic calli through Agrobacterium-mediated genetic transformation was established and optimized. The Agrobacterium tumefaciens strain EHA105, harboring the plasmid pBI121, which contained the ß-glucuronidase (GUS) gene and...

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Principais autores: Meiping Li, Dan Wang, Xiaofei Long, Zhaodong Hao, Ye Lu, Yanwei Zhou, Ye Peng, Tielong Cheng, Jisen Shi, Jinhui Chen
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2022-03-01
Serier:Frontiers in Plant Science
Fag:
suspension culture
somatic embryogenesis
agrobacterium tumefaciens
GUS
liriodendron hybrid
Online adgang:https://www.frontiersin.org/articles/10.3389/fpls.2022.802128/full
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