Validation of a Novel Double Control Quantitative Copy Number PCR Method to Quantify Off-Target Transgene Integration after CRISPR-Induced DNA Modification

In order to improve a recently established cell-based assay to assess the potency of botulinum neurotoxin, neuroblastoma-derived SiMa cells and induced pluripotent stem-cells (iPSC) were modified to incorporate the coding sequence of a reporter luciferase into a genetic safe harbor utilizing CRISPR/...

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Bibliografski detalji

Glavni autori:	Brit-Maren Michaud Schjeide, Maren Schenke, Bettina Seeger, Gerhard Paul Püschel
Format:	Artigo
Jezik:	Inglês
Izdano:	MDPI AG 2022-05-01
Serija:	Methods and Protocols
Teme:	CRISPR editing validation copy number analyses homology-directed repair homologous recombination deficiency
Online pristup:	https://www.mdpi.com/2409-9279/5/3/43
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