A comparative analysis of library prep approaches for sequencing low input translatome samples

Abstract Background Cell type-specific ribosome-pulldown has become an increasingly popular method for analysis of gene expression. It allows for expression analysis from intact tissues and monitoring of protein synthesis in vivo. However, while its utility has been assessed, technical aspects relat...

Полное описание

Библиографические подробности

Главные авторы:	Yang Song, Beatrice Milon, Sandra Ott, Xuechu Zhao, Lisa Sadzewicz, Amol Shetty, Erich T. Boger, Luke J. Tallon, Robert J. Morell, Anup Mahurkar, Ronna Hertzano
Формат:	Artigo
Язык:	Inglês
Опубликовано:	BMC 2018-09-01
Серии:	BMC Genomics
Предметы:	RiboTag Library preparation kits Low-input RNA-seq RNA-seq Coverage bias
Online-ссылка:	http://link.springer.com/article/10.1186/s12864-018-5066-2
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