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Application of Amplicon Metagenomics to Identify Fungal Pathogens in Formalin-Fixed Paraffin-Embedded Samples: Proof of Concept in Animals with Fungal Pathologies

The identification of fungal pathogens in formalin-fixed paraffin-embedded (FFPE) tissues is an unmet need in human and animal medicine, and sequence-agnostic approaches are needed to identify emerging pathogens. Eleven FFPE biopsy specimens with etiologic diagnoses of fungal disease based on standa...

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Autori principali: David B. Needle, Guillaume Reboul, Patrick K. Mitchell, Derek Rothenheber, Nicholas J. Marra, Brittany D. Cronk, Neeti G. Patel, Laura B. Goodman
Natura: Artigo
Lingua:Inglês
Pubblicazione: MDPI AG 2025-02-01
Serie:Microorganisms
Soggetti:
fungal diagnosis
molecular diagnostics
metabarcoding
FFPE sequencing
bioinformatics
Accesso online:https://www.mdpi.com/2076-2607/13/3/533
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