Generating stable isolated mitochondrial recipient clones in mammalian cells using MitoPunch mitochondrial transfer

Summary: This protocol describes the assembly and use of MitoPunch to deliver mitochondria containing mitochondrial DNA (mtDNA) into cells lacking mtDNA (ρ0 cells). MitoPunch generates stable isolated mitochondrial recipient clones with restored mtDNA and recovered respiration, enabling investigatio...

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Bibliografiske detaljer

Principais autores:	Alexander J. Sercel, Alexander J. Napior, Alexander N. Patananan, Ting-Hsiang Wu, Pei-Yu Chiou, Michael A. Teitell
Format:	Artigo
Sprog:	Inglês
Udgivet:	Elsevier 2021-12-01
Serier:	STAR Protocols
Fag:	Cell Biology Cell culture Cell-based Assays Metabolism Biotechnology and bioengineering
Online adgang:	http://www.sciencedirect.com/science/article/pii/S2666166721005566
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