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Use of the comet-FISH assay to compare DNA damage and repair in p53 and hTERT genes following ionizing radiation.

The alkaline single cell gel electrophoresis (comet) assay can be combined with fluorescent in situ hybridisation (FISH) methodology in order to investigate the localisation of specific gene domains within an individual cell. The number and position of the fluorescent signal(s) provides information...

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Autors principals: Declan J McKenna, Bernadette A Doherty, C Stephen Downes, Stephanie R McKeown, Valerie J McKelvey-Martin
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science (PLoS) 2012-01-01
Col·lecció:PLoS ONE
Accés en línia:http://europepmc.org/articles/PMC3492288?pdf=render
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