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CRISPR/Cas9 RET Gene Knockout in Medullary Thyroid Carcinoma Cell-lines: Optimization and Validation

Background: Medullary Thyroid Cancer (MTC) is a very aggressive type of thyroid carcinoma. Mutation in RET proto-oncogene is demonstrated in MTC development. We aimed to knock-out of RET-oncogene using CRISPR/Cas9 genome editing method in MTC cell-lines. Methods: This research was conducted in S...

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Principais autores: Marjan Zarif-Yeganeh, Dariush D Farhud, Azam Rahimpour, Sara Sheikholeslami, Setareh Shivaei, Mehdi Hedayati
Formato: Artigo
Idioma:Inglês
Publicado em: Tehran University of Medical Sciences 2022-05-01
coleção:Iranian Journal of Public Health
Assuntos:
CRISPR/Cas9
Gene editing
Medullary thyroid cancer
RET gene
TT cell-line
MZ-CRC-1 cell-line
Acesso em linha:https://ijph.tums.ac.ir/index.php/ijph/article/view/28675
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