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Translational efficiency of EMCV IRES in bicistronic vectors is dependent upon IRES sequence and gene location

The internal ribosomal entry site (IRES) from encephalomyocarditis virus (EMCV) is a popular RNA element used widely in experimental and pharmaceutical applications to express proteins in eukaryotic cells or cell-free extracts. Inclusion of the wild-type element in monocistronic or bicistronic messe...

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Bibliographic Details
Main Authors: Yury A. Bochkov, Ann C. Palmenberg
Format: Artigo
Language:Inglês
Published: Taylor & Francis Group 2006-09-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/000112243
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