13C Kinetic Labeling and Extraction of Metabolites from Adherent Mammalian Cells
Fluctuations in metabolite levels in mammalian cells are the most direct form of readout of the cellular metabolic state. The current protocol describes a method for pulse labeling and subsequent isolation of metabolites from adherent mammalian cells. The isolated metabolites can be identified and q...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Bio-protocol LLC
2015-04-01
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| Col·lecció: | Bio-Protocol |
| Accés en línia: | https://bio-protocol.org/en/bpdetail?id=1447&type=0 |
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