Global mapping of translation initiation sites by TIS profiling in budding yeast

Summary: Translation initiation site (TIS) profiling allows for the genome-wide identification of TISs in vivo by exclusively capturing mRNA fragments within ribosomes that have just completed translation initiation. It leverages translation inhibitors, such as harringtonine and lactimidomycin (LTM)...

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Ina Hollerer, Emily N. Powers, Gloria A. Brar
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Elsevier 2021-03-01
Schriftenreihe:	STAR Protocols
Schlagworte:	Cell biology Genomics High-throughput screening Model organisms Molecular biology
Online-Zugang:	http://www.sciencedirect.com/science/article/pii/S2666166720302379
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