Study of in vitro transcriptional binding effects and noise using constitutive promoters combined with UP element sequences in Escherichia coli

Abstract Background UP elements (upstream element) are DNA sequences upstream of a promoter that interact with the α-subunit of RNA polymerase (RNAP) and can affect transcription by altering the binding RNAP to DNA. However, details of UP element and binding affinity effects on transcriptional stren...

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Detalhes bibliográficos

Principais autores:	Qiang Yan, Stephen S. Fong
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2017-11-01
coleção:	Journal of Biological Engineering
Assuntos:	Upstream element sequence RNAP α subunits Gene expression noise Promoter strength RNA polymerase Binding association constant
Acesso em linha:	http://link.springer.com/article/10.1186/s13036-017-0075-2
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