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Use of a cysteine proteinase from Carica candamarcensis as a protective agent during DNA extraction

We describe the use of a plant cysteine proteinase isolated from latex of Carica candamarcensis as a protective agent during isolation of bacterial DNA following growth in culture of these cells. Between 100 to 720 units of proteinase (1 µg = 6 units) afforded good DNA protection when incubated with...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser: M.S. Genelhu, M.S. Zanini, I.F. Veloso, A.M.D. Carneiro, M.T.P. Lopes, C.E. Salas
Format: Artigo
Sprache:Inglês
Veröffentlicht: Associação Brasileira de Divulgação Científica 1998-09-01
Schriftenreihe:Brazilian Journal of Medical and Biological Research
Schlagworte:
cysteine proteinase
proteinase K
C. candamarcensis
DNA extraction
Online-Zugang:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900005
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