SYBR Green I实时荧光定量方法检测结直肠癌患者外周血CEA-mRNA表达研究
目的建立检测CEA mRNA表达水平的实时荧光定量逆转录聚合酶链反应（FQ-PCR）方法,探讨外周血CEA mRNA表达在结直肠癌患者中的应用价值。方法应用SYBR Green I作为荧光染料,以GAPDH作为内对照,建立检测CEA mRNA的FQ-PCR方法,并对56例结直肠癌患者外周血中CEA mRNA的表达水平进行了检测。结果56例结直肠癌患者中有33例外周血CEA mRNA表达为阳性,表达水平为1.07±2.99,阳性表达率为66.1%（37/56）,30例结直肠良性疾病患者中有2例外周血CEA mRNA表达为阳性,表达水平为0.13±0.07,阳性表达率为6.7%（2/30）,30例...
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| Principais autores: | , , , , |
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| Format: | Artigo |
| Jezik: | Chinês |
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Editorial Office of Chinese Journal of Laboratory Diagnosis
2007-01-01
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| Serija: | Zhongguo shiyan zhenduanxue |
| Teme: | |
| Online dostop: | http://ZSZD.publish.founderss.cn/thesisDetails?columnId=88545297&Fpath=home&index=0 |
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