Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering

The CRISPR-Cas9 technology represents a powerful tool for genome engineering in eukaryotic cells, advancing both fundamental research and therapeutic strategies. Despite the enormous potential of the technology, efficient and direct intracellular delivery of Cas9 ribonucleoprotein (RNP) complexes in...

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Detalhes bibliográficos

Principais autores:	Laurens Raes, Melissa Pille, Aranit Harizaj, Glenn Goetgeluk, Jelter Van Hoeck, Stephan Stremersch, Juan C. Fraire, Toon Brans, Olivier Gerrit de Jong, Roel Maas-Bakker, Enrico Mastrobattista, Pieter Vader, Stefaan C. De Smedt, Bart Vandekerckhove, Koen Raemdonck, Kevin Braeckmans
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2021-09-01
coleção:	Molecular Therapy: Nucleic Acids
Assuntos:	CRISPR-Cas9 gene editing T cells stem cells photoporation intracellular delivery
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2162253121002080
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