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Comparison and evaluation of metagenomic next-generation sequencing (mNGS) and real-time PCR for the detection of Mycobacterium tuberculosis

ObjectiveThis study aimed to evaluate and compare the performance of metagenomic next-generation sequencing (mNGS) and real-time polymerase chain reaction (RT-PCR) for the detection of Mycobacterium tuberculosis (MTB) in patients with suspected tuberculosis (TB).MethodsSamples from patients undergoi...

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Principais autores: Yanhong Wang, Penghao Guo, Yaoming Chen, Hongji Zhu, Xuegao Yu, Jiankai Deng
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2025-11-01
Serier:Frontiers in Cellular and Infection Microbiology
Fag:
metagenomic next-generation sequencing
real-time PCR
tuberculosis diagnosis
pulmonary tuberculosis
extrapulmonary tuberculosis
Online adgang:https://www.frontiersin.org/articles/10.3389/fcimb.2025.1694179/full
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