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Protocol for mapping the heterogeneous dendritic cell network across the murine tissue landscape via high-dimensional flow cytometry

Summary: Dendritic cells (DCs) populate nearly all tissues and represent the central orchestrators of immunity. Here, we present a protocol for the mild but efficient preparation of single-cell suspensions from multiple murine tissues and the downstream analysis of the DC network via high-parameter...

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Principais autores: Lukas Amon, Anna Seichter, Damir Vurnek, Giorgi Tchitashvili, Isabel Heß, Lukas Heger, Christian H.K. Lehmann, Diana Dudziak
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2024-09-01
Serier:STAR Protocols
Fag:
Cell Biology
Cell isolation
Single Cell
Flow Cytometry
Immunology
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166724003162
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