Fluorescence lifetime FRET assay for live-cell high-throughput screening of the cardiac SERCA pump yields multiple classes of small-molecule allosteric modulators

Abstract We have used FRET-based biosensors in live cells, in a robust high-throughput screening (HTS) platform, to identify small-molecules that alter the structure and activity of the cardiac sarco/endoplasmic reticulum calcium ATPase (SERCA2a). Our primary aim is to discover drug-like small-molec...

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Principais autores:	Osha Roopnarine, Samantha L. Yuen, Andrew R. Thompson, Lauren N. Roelike, Robyn T. Rebbeck, Philip A. Bidwell, Courtney C. Aldrich, Razvan L. Cornea, David D. Thomas
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Nature Portfolio 2023-07-01
coleção:	Scientific Reports
Acesso em linha:	https://doi.org/10.1038/s41598-023-37704-x
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