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Protocol for the derivation, culturing, and differentiation of human iPS-cell-derived neuroepithelial stem cells to study neural differentiation in vitro

Summary: Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent stem cells. NES cells can be further differentiated into a culture of neurons (90%) and glia (10%). We describe how to derive and maintain NES cells in culture and how to differenti...

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Principais autores: Javier Calvo-Garrido, Dania Winn, Camilla Maffezzini, Anna Wedell, Christoph Freyer, Anna Falk, Anna Wredenberg
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021-06-01
coleção:STAR Protocols
Assuntos:
Neuroscience
Stem Cells
Cell Differentiation
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S2666166721002355
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