Developing the script “degenerate primer 111” to enhance the coverage of universal primers for the small subunit rRNA gene on target microorganisms

Amplifying small subunit (SSU) rRNA genes with universal primers in assessing microbial populations diversity, but target microorganisms are sometimes omitted due to inadequate primer coverage. Adding degenerate bases to primers can help, but existing methods are complex and time-consuming. This stu...

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Bibliografische Detailangaben

Hauptverfasser:	Zhihui Qin, Xin Xu, Fengjun Xu, Yao Zhang, Peng Su, Chaofeng Shen
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Frontiers Media S.A. 2024-09-01
Schriftenreihe:	Frontiers in Microbiology
Schlagworte:	16S rRNA 18S rRNA bacteria archaea eukaryote high-throughput sequencing
Online-Zugang:	https://www.frontiersin.org/articles/10.3389/fmicb.2024.1394303/full
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