Mapping the membrane orientation of auxin homeostasis regulators PIN5 and PIN8 in Arabidopsis thaliana root cells reveals their divergent topology

Abstract PIN proteins establish the auxin concentration gradient, which coordinates plant growth. PIN1-4 and 7 localized at the plasma membrane (PM) and facilitate polar auxin transport while the endoplasmic reticulum (ER) localized PIN5 and PIN8 maintain the intracellular auxin homeostasis. Althoug...

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Hlavní autoři:	Yewubnesh Wendimu Seifu, Vendula Pukyšová, Nikola Rýdza, Veronika Bilanovičová, Marta Zwiewka, Marek Sedláček, Tomasz Nodzyński
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	BMC 2024-06-01
Edice:	Plant Methods
Témata:	Arabidopsis thaliana Auxin homeostasis and transport Digitonin PM-permeabilizing protocols Endoplasmic reticulum Immunocytochemistry Membrane topology determination methods
On-line přístup:	https://doi.org/10.1186/s13007-024-01182-7
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