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Superior normalization using total protein for western blot analysis of human adipocytes.

Western blotting has been a pivotal method for analyzing protein expression since the late 1970s, yet there is no established consensus on an optimal normalization strategy. In this study, primary mature human adipocytes were used to investigate the robustness of housekeeping proteins and total prot...

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Autori principali: Leo J S Westerberg, Benjamin Dedic, Erik Näslund, Anders Thorell, Kirsty L Spalding
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science (PLoS) 2025-01-01
Serie:PLoS ONE
Accesso online:https://doi.org/10.1371/journal.pone.0328136
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