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Evaluation of acrosomal status and sperm viability in fresh and cryopreserved specimens by the use of fluorescent peanut agglutinin lectin in conjunction with hypo-osmotic swelling test

OBJECTIVE: In this study, we evaluated whether the hypo-osmotic swelling test (HOST) can be used as a vital marker in combination with peanut agglutinin (PNA) - labeling in fresh and cryopreserved spermatozoa. MATERIALS AND METHODS: Human sperm populations were exposed to a hypo-osmotic medium for 6...

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Autors principals: Sandro C. Esteves, Rakesh K. Sharma, Anthony J. Thomas Jr, Ashok Agarwal
Format: Artigo
Idioma:Inglês
Publicat: Sociedade Brasileira de Urologia 2007-06-01
Col·lecció:International Brazilian Journal of Urology
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Accés en línia:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382007000300009
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