A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR.
Non-specific tail sequences are often added to the 5'-terminus of primers to improve the robustness and overall performance of diagnostic assays. Despite the widespread use of tailed primers, the underlying working mechanism is not well understood. To address this problem, we conducted a detailed in...
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| Principais autores: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Acceso en liña: | http://europepmc.org/articles/PMC5056738?pdf=render |
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