Protocol for isolating human BCAM-positive corneal progenitor cells by flow cytometry and cell sorting

Summary: BCAM-positive basal limbal epithelial cells are an early transit-amplifying cell population (TAC) capable of holoclone formation and corneal epithelial differentiation. Here, we present a protocol for isolating BCAM-positive cells from human donor corneas by flow cytometry and cell sorting....

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Auteurs principaux:	Yuzuru Sasamoto, Philip C. Yeung, Johnathan Tran, Markus H. Frank, Natasha Y. Frank
Format:	Artigo
Langue:	Inglês
Publié:	Elsevier 2023-09-01
Collection:	STAR Protocols
Sujets:	Cell Biology Cell Isolation Flow Cytometry/Mass Cytometry Cell-based Assays Stem Cells
Accès en ligne:	http://www.sciencedirect.com/science/article/pii/S2666166723004707
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