Live cell imaging and proteomic profiling of endogenous NEAT1 lncRNA by CRISPR/Cas9-mediated knock-in

Abstract In mammalian cells, long noncoding RNAs (lncRNAs) form complexes with proteins to execute various biological functions such as gene transcription, RNA processing and other signaling activities. However, methods to track endogenous lncRNA dynamics in live cells and screen for lncRNA interact...

पूर्ण विवरण

ग्रंथसूची विवरण

मुख्य लेखकों:	Bohong Chen, Shengcheng Deng, Tianyu Ge, Miaoman Ye, Jianping Yu, Song Lin, Wenbin Ma, Zhou Songyang
स्वरूप:	Artigo
भाषा:	Inglês
प्रकाशित:	Oxford University Press 2020-05-01
श्रृंखला:	Protein & Cell
विषय:	CRISPR/Cas9 genome editing endogenous lncRNA labeling MS2-MCP NEAT1 paraspeckle dynamics
ऑनलाइन पहुंच:	https://doi.org/10.1007/s13238-020-00706-w
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