Protocol for expressing and purifying recombinant full-length Tau by combining affinity chromatography with preparative HPLC

Summary: Deposits of microtubule-associated protein full-length Tau (Tau2N4R) are implicated as a hallmark of Alzheimer’s disease. Its biochemical and structural characterization is key to understanding disease progression, aggregate toxicity, and designing therapeutics. We present a protocol for ex...

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Hauptverfasser:	Ruiheng Jing, Sayanta Mahapatra, Abhishek Bimali, Morgane Herlory, Maciej A. Walczak
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Elsevier 2026-09-01
Schriftenreihe:	STAR Protocols
Schlagworte:	Biophysics Protein expression and purification Mass Spectrometry
Online-Zugang:	http://www.sciencedirect.com/science/article/pii/S2666166726002704
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