A CRISPR/Cas9-Based Mutagenesis Protocol for Brachypodium distachyon and Its Allopolyploid Relative, Brachypodium hybridum

The CRISPR/Cas9 system enables precise genome editing and is a useful tool for functional genomic studies. Here we report a detailed protocol for targeted genome editing in the model grass Brachypodium distachyon and its allotetraploid relative B. hybridum, describing gRNA design, a transient protop...

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主要な著者:	Karolina Hus, Alexander Betekhtin, Artur Pinski, Magdalena Rojek-Jelonek, Ewa Grzebelus, Candida Nibau, Mingjun Gao, Katja E. Jaeger, Glyn Jenkins, John H. Doonan, Robert Hasterok
フォーマット:	Artigo
言語:	Inglês
出版事項:	Frontiers Media S.A. 2020-05-01
シリーズ:	Frontiers in Plant Science
主題:	CRISPR/Cas9 system targeted mutagenesis Brachypodium distachyon Brachypodium hybridum Agrobacterium-mediated transformation transient protoplast assay
オンライン･アクセス:	https://www.frontiersin.org/article/10.3389/fpls.2020.00614/full
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