A Non‐Centrifugation Method to Concentrate and Purify Extracellular Vesicles Using Superabsorbent Polymer Followed by Size Exclusion Chromatography

ABSTRACT Extracellular vesicles (EVs) can be isolated and purified from cell cultures and biofluids using different methodologies. Here, we explored a novel EV isolation approach by combining superabsorbent polymers (SAP) in a dialysis membrane with size exclusion chromatography (SEC) to achieve hig...

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Principais autores:	Markus Bergqvist, Cecilia Lässer, Rossella Crescitelli, Kyong‐Su Park, Jan Lötvall
Formato:	Artigo
Idioma:	Inglês
Publicado:	Wiley 2025-01-01
Series:	Journal of Extracellular Vesicles
Assuntos:	exosomes EV isolation techniques non‐ultracentrifugation methods size exclusion chromatography superabsorbent polymers Thermo Luciferase
Acceso en liña:	https://doi.org/10.1002/jev2.70037
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