Mechanism and application of Taq DNA polymerase in TaqMan qPCR

TaqMan quantitative real-time PCR (qPCR) is widely used in molecular biology due to its high specificity, sensitivity, and multiplexing capability. Taq DNA polymerase (Taq pol) plays a central role by simultaneously catalyzing DNA synthesis and cleaving fluorogenic probes, yet the mechanistic interp...

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Номзүйн дэлгэрэнгүй

Үндсэн зохиолчид:	Qiuyu Cao, Ziling Zou, Duanru Cao, Xiaofei Zhang, Tao Shu, Zhipeng Qu, Yanchang Zhao
Формат:	Artigo
Хэл сонгох:	Inglês
Хэвлэсэн:	Frontiers Media S.A. 2026-05-01
Цуврал:	Frontiers in Bioengineering and Biotechnology
Нөхцлүүд:	nuclease activity probe cleavage efficiency probe melting curve Taq pol TaqMan qPCR
Онлайн хандалт:	https://www.frontiersin.org/articles/10.3389/fbioe.2026.1773703/full
Шошгууд:	Шошго нэмэх Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!