SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase.
SpyTag can spontaneously form a covalent isopeptide bond with its protein partner SpyCatcher. Firefly luciferase from Photinus pyralis was cyclized in vivo by fusing SpyCatcher at the N terminus and SpyTag at the C terminus. Circular LUC was more thermostable and alkali-tolerant than the wild type,...
Αποθηκεύτηκε σε:
| Κύριοι συγγραφείς: | , , , |
|---|---|
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
Public Library of Science (PLoS)
2016-01-01
|
| Σειρά: | PLoS ONE |
| Διαθέσιμο Online: | http://europepmc.org/articles/PMC5033358?pdf=render |
| Ετικέτες: |
Δεν υπάρχουν, Καταχωρήστε ετικέτα πρώτοι!
|