Protocol to model tumor hypoxia in vitro using real-time phosphorescence-based sensing of O2 gradients generated by metastatic cancer cells

Summary: Tumor hypoxia plays a critical role in cancer progression and therapeutic resistance. Here, we present a protocol for the self-generation of hypoxia by metastatic cancer cells using phosphorescence-based O2 sensing. We describe steps for phosphorescent film calibration, system assembly, and...

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Detalhes bibliográficos

Principais autores:	Noreen Hosny, Kimberly Shen, Yihua Zhao, Junle Qu, Yusha Sun, George Butler, Sarah Amend, Emma U. Hammarlund, Robert Gatenby, Joel Brown, Kenneth J. Pienta, Trung V. Phan, Stephano Boyer-Paulet, Shengkai Li, Robert H. Austin
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2025-12-01
coleção:	STAR Protocols
Assuntos:	Cell Biology Cancer Biotechnology and bioengineering
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166725005696
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