Establishment and Application of Real-time Fluorescence Single Primer Isothermal Amplification for Shigella(志贺氏菌实时荧光单引物等温扩增方法的建立及应用)

Based on the Shigella ipaH gene, the RNA-DNA primers and Blockers were designed and synthetized, and the real-time fluorescence single primer isothermal amplification (real-time fluorescence SPIA) for the detection of Shigella was established. The reaction time of the method was 44min. After detecti...

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Detalles Bibliográficos

Principais autores:	WANG Jianchang(王建昌), HU Lianxia(胡连霞), DUAN Yongsheng(段永生), LI Jing(李 静), WANG Jinfeng(王金凤)
Formato:	Artigo
Idioma:	Chinês
Publicado:	Beijing Technology and Business University 2015-11-01
Series:	Shipin kexue jishu xuebao
Assuntos:	real-time fluorescence single primer isothermal amplification(实时荧光单引物等温扩增) shigella( 志贺氏菌) ipah gene
Acceso en liña:	https://www.btbuspxb.com/spkxjsxb/article/pdf/20150607
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