Generation of a Novel High-Affinity Antibody Binding to PCSK9 Catalytic Domain with Slow Dissociation Rate by CDR-Grafting, Alanine Scanning and Saturated Site-Directed Mutagenesis for Favorably Treating Hypercholesterolemia

Inhibition of proprotein convertase subtilisin/kexin type 9 (PCSK9) has become an attractive therapeutic strategy for lowering low-density lipoprotein cholesterol (LDL-C). In this study, a novel high affinity humanized IgG1 mAb (named h5E12-L230G) targeting the catalytic domain of human PCSK9 (hPCSK...

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Principais autores:	Zhengli Bai, Menglong Xu, Ying Mei, Tuo Hu, Panpan Zhang, Manman Chen, Wenxiu Lv, Chenchen Lu, Shuhua Tan
Formato:	Artigo
Idioma:	Inglês
Publicado em:	MDPI AG 2021-11-01
coleção:	Biomedicines
Assuntos:	single-chain variable fragment (scFv) alanine scanning saturated site-directed mutagenesis PCSK9 molecular docking slow dissociation rate
Acesso em linha:	https://www.mdpi.com/2227-9059/9/12/1783
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