Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation

Abstract Staphylococcus aureus Cas9 (SaCas9) is smaller than the widely used Streptococcus pyogenes Cas9 (SpCas9) and has been harnessed for gene therapy using an adeno-associated virus vector. However, SaCas9 requires a longer NNGRRT (where N is any nucleotide and R is A or G) protospacer adjacent...

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Principais autores:	Satoshi N. Omura, Ryoya Nakagawa, Shohei Kajimoto, Sae Okazaki, Soh Ishiguro, Hideto Mori, Kosuke Onishi, Yuji Kashiwakura, Takafumi Hiramoto, Kio Horinaka, Mamoru Tanaka, Hisato Hirano, Kasey Jividen, Keitaro Yamashita, Shengdar Q. Tsai, Nozomu Yachie, Tsukasa Ohmori, Hiroshi Nishimasu, Osamu Nureki
格式:	Artigo
語言:	Inglês
出版:	Nature Portfolio 2026-04-01
叢編:	Nature Communications
在線閱讀:	https://doi.org/10.1038/s41467-026-71626-2
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