A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable.

Aspergillus niger and other filamentous fungi are widely used in industry, but efficient genetic engineering of these hosts remains nascent. For example, while molecular genetic tools have been developed, including CRISPR/Cas9, facile genome engineering of A. niger remains challenging. To address th...

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Autors principals:	Laure M C Leynaud-Kieffer, Samuel C Curran, Irene Kim, Jon K Magnuson, John M Gladden, Scott E Baker, Blake A Simmons
Format:	Artigo
Idioma:	Inglês
Publicat:	Public Library of Science (PLoS) 2019-01-01
Col·lecció:	PLoS ONE
Accés en línia:	https://doi.org/10.1371/journal.pone.0210243
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