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Increased cytoplasmic expression of PETase enzymes in E. coli

Abstract Background Depolymerizing polyethylene terephthalate (PET) plastics using enzymes, such as PETase, offers a sustainable chemical recycling route. To enhance degradation, many groups have sought to engineer PETase for faster catalysis on PET and elevated stability. Considerably less effort h...

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Principais autores: Luke M. Carter, Chris E. MacFarlane, Samuel P. Karlock, Tridwip Sen, Joel L. Kaar, Jason A. Berberich, Jason T. Boock
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2024-11-01
Serier:Microbial Cell Factories
Fag:
PETase
Protein expression
Protein purification
SHuffle
Bioreactor
Online adgang:https://doi.org/10.1186/s12934-024-02585-w
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