Manipulating mRNA-binding protein Cth2 function in budding yeast Saccharomyces cerevisiae

Summary: Here, we present a protocol for modulating the function of the Cth2 mRNA-binding protein (RBP) in Saccharomyces cerevisiae. We describe steps to amplify and integrate mutations in Cth2 that affect its stability and function. Next, we detail the functional assay to verify the activity of the...

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Bibliografske podrobnosti

Principais autores:	Praveen K. Patnaik, Hanna Barlit, Vyacheslav M. Labunskyy
Format:	Artigo
Jezik:	Inglês
Izdano:	Elsevier 2024-03-01
Serija:	STAR Protocols
Teme:	Cell-based Assays Model Organisms Molecular Biology Gene Expression
Online dostop:	http://www.sciencedirect.com/science/article/pii/S2666166723007748
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