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Effect of pH and Ionic Strength on the Spectrophotometric Assessment of Nucleic Acid Purity

The ratio of absorbance at 260 and 280 nm (the A260/280 ratio) is frequently used to assess the purity of RNA and DNA preparations. Data presented in this report demonstrate significant variability in the RNA A260/280 ratio when different sources of water were used to perform the spectrophotometric...

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Autors principals: William W. Wilfinger, Karol Mackey, Piotr Chomczynski
Format: Artigo
Idioma:Inglês
Publicat: Taylor & Francis Group 1997-03-01
Col·lecció:BioTechniques
Accés en línia:https://www.future-science.com/doi/10.2144/97223st01
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