Single-organelle DNA-sequencing of chloroplasts and mitochondria in Arabidopsis thaliana

Abstract Organelle genomes govern bioenergetics and stress responses, yet bulk sequencing masks heterogeneity. We establish single-organelle DNA-sequencing by adapting a droplet-based workflow to mitochondria and chloroplasts, using a sorbitol-based isolation that preserves organelles for encapsulat...

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Auteurs principaux:	Zikai Xiang, Kazuki Takahashi, Chang Zhou, Hideki Takanashi, Shin-ichi Arimura, Masahito Hosokawa
Format:	Artigo
Langue:	Inglês
Publié:	BMC 2026-01-01
Collection:	BMC Plant Biology
Sujets:	Single-organelle sequencing Arabidopsis thaliana Chloroplast Mitochondria
Accès en ligne:	https://doi.org/10.1186/s12870-026-08232-3
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