Ratio determination of plasma wild-type and L159R apoA-I using mass spectrometry: tools for studying apoA-IFin

In this report, methods are described to isolate milligram quantities of a mutant apolipoprotein A-I (apoA-I) protein for use in structure-function studies. Expression of the L159R apoA-I mutation in humans reduces the concentration of plasma wild-type apoA-I, thus displaying a dominant negative phe...

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Autori principali:	John S. Owen, Manish S. Bharadwaj, Michael J. Thomas, Shaila Bhat, Michael P. Samuel, Mary G. Sorci-Thomas
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Elsevier 2007-01-01
Serie:	Journal of Lipid Research
Soggetti:	apolipoprotein A-I protein expression inclusion bodies apolipoprotein A-I mutant high-performance liquid chromatography-electrospray mass spectrometry high-performance liquid chromatography-electrospray tandem mass spectrometry
Accesso online:	http://www.sciencedirect.com/science/article/pii/S0022227520436545
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