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Screening of cis-acting elements for maintaining pluripotency of human embryonic stem cells

Objective To establish an inducible CRISPR library screening method for human embryonic stem cells (hESCs) to screen the cis-acting elements involed in the maintainance the pluripotency of hESCs. Methods The inducible Cas9 expression elements were inserted into the hESC genome to construct an indu...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
מחבר ראשי: SUN Meng-yao, LIU Si-qi, ZHOU Fan-qi, MA Yan-ni, YU Jia
פורמט: Artigo
שפה:Chinês
יצא לאור: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. 2021-05-01
סדרה:Jichu yixue yu linchuang
נושאים:
גישה מקוונת:http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210082.pdf
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