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Universal stem-loop primer method for screening and quantification of microRNA.

RT-qPCR is the accepted technique for the quantification of microRNA (miR) expression: however, stem-loop RT-PCR, the most frequently used method for quantification of miRs, is time- and reagent-consuming as well as inconvenient for scanning. We established a new method called 'universal stem-loop p...

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Principais autores: Li-hong Yang, Si-lu Wang, Li-li Tang, Biao Liu, Wen-le Ye, Ling-ling Wang, Zhang-yang Wang, Meng-tao Zhou, Bi-cheng Chen
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science (PLoS) 2014-01-01
Serier:PLoS ONE
Online adgang:https://doi.org/10.1371/journal.pone.0115293
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