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Serum coating enables feeder-free culture of naive human pluripotent stem cells preserving developmental potential

Abstract Naive human pluripotent stem cells (hPSCs) represent a pre-implantation epiblast state able to efficiently differentiate into embryonic and extraembryonic pre-implantation lineages and to self-organise in vitro into blastocyst-like structures called blastoids. Naive hPSC maintenance routine...

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Principais autores: Giada Rossignoli, Michael Oberhuemer, Ida Sophie Brun, Irene Zorzan, Anna Osnato, Anne Wenzel, Emiel van Genderen, Andrea Drusin, Giorgia Panebianco, Nicolò Magri, Moritz Becker, Mairim Alexandra Solis, Chiara Colantuono, Sam Samuël Franciscus Allegonda van Knippenberg, Thi Xuan Ai Pham, Sherif Khodeer, Paolo Grumati, Davide Cacchiarelli, Paolo Martini, Nicolas Rivron, Vincent Pasque, Jan Jakub Żylicz, Martin Leeb, Graziano Martello
Formato: Artigo
Idioma:Inglês
Publicado: Springer Nature 2026-03-01
Series:The EMBO Journal
Assuntos:
Human Naive Pluripotent Stem Cells
Blastoids
Extraembryonic Lineages
Extracellular Matrix
Feeder-free
Acceso en liña:https://doi.org/10.1038/s44318-026-00714-2
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