Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Coconversion

Adoption of a streamlined version of the bacterial clustered regular interspersed short palindromic repeat (CRISPR)/Cas9 defense system has accelerated targeted genome engineering. The Streptococcus pyogenes Cas9 protein, directed by a simplified, CRISPR-like single-guide RNA, catalyzes a double-str...

ver descrição completa

Detalhes bibliográficos

Principais autores:	Daniel Tianfang Ge, Cindy Tipping, Michael H. Brodsky, Phillip D. Zamore
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2016-10-01
coleção:	G3: Genes, Genomes, Genetics
Assuntos:	Cas9 coconversion genetic screen microhomology-mediated end joining homologous recombination
Acesso em linha:	http://g3journal.org/lookup/doi/10.1534/g3.116.032557
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!