Use of Manganese in RT-PCR Eliminates PCR Artifacts Resulting from DNase I Digestion
The precise quantification of rare mRNA copies from intronless genes by reverse transcription polymerase chain reaction (RT-PCR) requires the complete removal of genomic DNA because discrimination of cDNA and DNA amplification products by differing sizes of PCR products is not possible. Elimination...
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| Автори: | , , , , |
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| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Taylor & Francis Group
1997-06-01
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| Серія: | BioTechniques |
| Онлайн доступ: | https://www.future-science.com/doi/10.2144/97226st05 |
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