Double Electroporation Combined with Zona Pellucida Removal Improves Biallelic Genome Editing Efficiency in Porcine Embryos

The CRISPR/Cas9 system has been widely used for gene editing in various species; however, mosaicism remains a significant challenge. This study aimed to improve gene editing efficiency and reduce mosaicism in porcine embryos by exploring double electroporation pre- and post-in vitro fertilization co...

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Auteurs principaux:	Nanaka Torigoe, Takeshige Otoi, Manita Wittayarat, Oky Setyo Widodo, Theerawat Tharasanit, Kaywalee Chatdarong, Megumi Nagahara, Maki Hirata, Fuminori Tanihara, Zhao Namula
Format:	Artigo
Langue:	Inglês
Publié:	MDPI AG 2026-06-01
Collection:	Animals
Sujets:	double electroporation gene editing mosaic mutation pig zona free
Accès en ligne:	https://www.mdpi.com/2076-2615/16/12/1919
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