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Reproducibility in the Quantification of mRNA Levels by RT-PCR-ELISA and RT Competitive-PCR-ELISA

The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps. Semi-competitive RT-PCR (RT-cPCR) uses an internal competitor template in the PCR step to improve quantitation. We have surve...

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Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: L.L. Hall, G.R. Bicknell, L. Primrose, J.H. Pringle, J.A. Shaw, P.N. Furness
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Taylor & Francis Group 1998-04-01
Saila:BioTechniques
Sarrera elektronikoa:https://www.future-science.com/doi/10.2144/98244rr02
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